8I4O

Design of a split green fluorescent protein for sensing and tracking an beta-amyloid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	110
Detector technology	PIXEL
Collection date	2022-03-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00003
Spacegroup name	P 1
Unit cell lengths	64.804, 68.819, 101.239
Unit cell angles	98.38, 97.97, 113.44

Refinement procedure

Resolution	33.940 - 3.100
R-factor	0.2562
Rwork	0.252
R-free	0.32340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.476
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	7.590
High resolution limit [Å]	2.800	2.800
Number of reflections	25720	35216
<I/σ(I)>	10.8
Completeness [%]	91.5
Redundancy	3.5
CC(1/2)	0.975	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100mM imidazole pH 8.0, 200mM Zn(OAc)2, 20% 1,4-butanediol