8I2K
Crystal structure of transcriptional regulator pvrA from Pseudomonas aeruginosa.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-10 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.5 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 93.298, 96.584, 104.789 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.368 - 2.303 |
| R-factor | 0.1975 |
| Rwork | 0.192 |
| R-free | 0.25400 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.233 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | AutoSol |
| Refinement software | PHENIX (v1.14) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.960 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 21122 | 2059 |
| <I/σ(I)> | 27.8 | |
| Completeness [%] | 99.2 | |
| Redundancy | 18.7 | |
| CC(1/2) | 0.999 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | PEG 3000, 100mM Tris, 200mM Nacl |
