8I11

Crystal structure of LOV1 domain of phototropin from Klebsormidium nitens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.88560
Spacegroup name	P 43 21 2
Unit cell lengths	53.868, 53.868, 137.014
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.340 - 1.855
Rwork	0.193
R-free	0.22080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.661
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.340	5.000
High resolution limit [Å]	1.850	1.855
Number of reflections	17804	858
<I/σ(I)>	17.7
Completeness [%]	99.7
Redundancy	6.8
CC(1/2)	0.990	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289.15	magnesium Sulfate hydrate, HEPES sodium pH 7.0, lithium sulfate monohydrate