8I0X

Beta-Xylosidase JB13GH39P28 showing salt/ethanol/trypsin tolerance, low-pH/low-Temperature activity, and transformation of notoginsenosides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	CCD
Collection date	2022-09-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	64.403, 62.627, 140.117
Unit cell angles	90.00, 91.42, 90.00

Refinement procedure

Resolution	32.640 - 2.000
R-factor	0.1707
Rwork	0.169
R-free	0.20960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.759
Data reduction software	DIALS (3.7.2)
Data scaling software	DIALS (3.7.2)
Phasing software	PHENIX (1.15.1_3469)
Refinement software	PHENIX (1.15.1_3469)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.640	2.071
High resolution limit [Å]	1.999	1.999
Rmeas	0.353
Rpim	0.139
Number of reflections	75588	7109
<I/σ(I)>	6.37	2.06
Completeness [%]	97.9	94.51
Redundancy	6.5	6.6
CC(1/2)	0.980	0.515

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291.15	16% PEG 3350,100 mM BIS-TRIS propane pH 8.5, 20.00 mM Sodium Potassium phosphate