8I0O
Crystal structure of Transthyretin variant A97S in monomeric form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-10-21 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.932, 83.970, 84.938 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.790 - 1.880 |
| R-factor | 0.1875 |
| Rwork | 0.185 |
| R-free | 0.23360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.079 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmeas | 0.129 | |
| Rpim | 0.040 | 0.324 |
| Number of reflections | 37066 | 3513 |
| <I/σ(I)> | 16.23 | |
| Completeness [%] | 94.0 | |
| Redundancy | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1 M Sodium HEPES; MOPS (acid), 0.3M Sodium fluoride; 0.3M Sodium bromide; 0.3M Sodium iodide, 25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350 |
