8HZM

A new fluorescent RNA aptamer bound with N, manganese soak

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.240
Spacegroup name	C 1 2 1
Unit cell lengths	77.694, 46.871, 56.722
Unit cell angles	90.00, 113.70, 90.00

Refinement procedure

Resolution	39.140 - 2.500
R-factor	0.2073
Rwork	0.205
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8hze
RMSD bond length	0.006
RMSD bond angle	1.142
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.116	0.115	0.561
Rmeas	0.126	0.125	0.655
Rpim	0.049	0.047	0.329
Total number of observations	37919
Number of reflections	6395	347	260
<I/σ(I)>	4.5
Completeness [%]	97.1	99.4	78.1
Redundancy	5.9	6.4	3.2
CC(1/2)	0.984	0.988	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.05 M Magnesium Chloride, 0.1 M HEPES pH7.5, 30%polyethylene glycol monomethyl ether 550, 0.6% 1,6-diaminohexane