8HZJ

A new fluorescent RNA aptamer bound with N571

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.102
Spacegroup name	C 1 2 1
Unit cell lengths	89.316, 48.173, 58.304
Unit cell angles	90.00, 116.03, 90.00

Refinement procedure

Resolution	29.370 - 2.600
R-factor	0.1919
Rwork	0.188
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8hze
RMSD bond length	0.005
RMSD bond angle	1.095
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.079	0.046	0.454
Rmeas	0.086	0.051	0.494
Rpim	0.033	0.020	0.191
Number of reflections	6872	357	346
<I/σ(I)>	4.5
Completeness [%]	98.6	96.2	96.1
Redundancy	6.7	6.4	6.3
CC(1/2)		0.999	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.08 M Sodium Chloride, 0.02 M Magnesium Chloride, 0.04 M Sodium Cacodylate trihydrate pH6.0, 35%MPD, 0.012 M Spermine tetrahydrochloride