8HZJ
A new fluorescent RNA aptamer bound with N571
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.102 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.316, 48.173, 58.304 |
Unit cell angles | 90.00, 116.03, 90.00 |
Refinement procedure
Resolution | 29.370 - 2.600 |
R-factor | 0.1919 |
Rwork | 0.188 |
R-free | 0.25870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8hze |
RMSD bond length | 0.005 |
RMSD bond angle | 1.095 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
Rmerge | 0.079 | 0.046 | 0.454 |
Rmeas | 0.086 | 0.051 | 0.494 |
Rpim | 0.033 | 0.020 | 0.191 |
Number of reflections | 6872 | 357 | 346 |
<I/σ(I)> | 4.5 | ||
Completeness [%] | 98.6 | 96.2 | 96.1 |
Redundancy | 6.7 | 6.4 | 6.3 |
CC(1/2) | 0.999 | 0.931 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.08 M Sodium Chloride, 0.02 M Magnesium Chloride, 0.04 M Sodium Cacodylate trihydrate pH6.0, 35%MPD, 0.012 M Spermine tetrahydrochloride |