8HZF
A new fluorescent RNA aptamer bound with N565
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1020 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 84.634, 47.237, 56.170 |
Unit cell angles | 90.00, 115.26, 90.00 |
Refinement procedure
Resolution | 40.200 - 2.700 |
R-factor | 0.186 |
Rwork | 0.182 |
R-free | 0.25580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8hze |
RMSD bond length | 0.006 |
RMSD bond angle | 1.370 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
Rmerge | 0.156 | 0.072 | 1.268 |
Rmeas | 0.169 | 0.079 | 1.376 |
Rpim | 0.065 | 0.031 | 0.528 |
Number of reflections | 5579 | 580 | 557 |
<I/σ(I)> | 2.8 | ||
Completeness [%] | 98.9 | 98.1 | 99.6 |
Redundancy | 6.7 | 6.4 | 6.7 |
CC(1/2) | 0.998 | 0.735 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M Bicine pH8.5, 65%MPD |