8HZD
A new fluorescent RNA aptamer bound with N618
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.102 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.494, 46.461, 56.251 |
Unit cell angles | 90.00, 113.81, 90.00 |
Refinement procedure
Resolution | 28.160 - 1.870 |
R-factor | 0.213 |
Rwork | 0.211 |
R-free | 0.25920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8hze |
RMSD bond length | 0.005 |
RMSD bond angle | 1.030 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.860 | 1.970 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.060 | 0.648 |
Rmeas | 0.065 | 0.713 |
Rpim | 0.026 | 0.279 |
Number of reflections | 14913 | 3566 |
<I/σ(I)> | 14.4 | |
Completeness [%] | 96.9 | |
Redundancy | 6.4 | |
CC(1/2) | 0.999 | 0.883 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.05 M Magnesium Chloride, 0.1 M HEPES pH7.5, 30%(w/v) PEGmm550, 0.001 M GSH |