8HYL
Crystal structure of DO1 Fv-clasp fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.899995 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.277, 86.207, 112.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.000 |
| R-factor | 0.2442 |
| Rwork | 0.241 |
| R-free | 0.29810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.316 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487: 000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.182 | 2.000 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 92507 | 14760 |
| <I/σ(I)> | 5.89 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3.38 | |
| CC(1/2) | 0.993 | 0.460 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.1 M Tris-HCl (pH 7.5), 25%[w/v] PEG 3350, 0.2 M lithium nitrate |
