8HXS

Small_spotted catshark CD8alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	291.15
Detector technology	CCD
Collection date	2020-08-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9979
Spacegroup name	C 1 2 1
Unit cell lengths	72.384, 40.446, 32.828
Unit cell angles	90.00, 92.56, 90.00

Refinement procedure

Resolution	35.324 - 1.350
Rwork	0.212
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.370
High resolution limit [Å]	1.350	1.350
Number of reflections	20802
<I/σ(I)>	2.19
Completeness [%]	99.2
Redundancy	12.4
CC(1/2)	0.960	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.5	30% (w/v) PEG3000 and 100mM CHES/Sodium hydroxide pH9.5