8HWJ
Bacterial STING from Epilithonimonas lactis in complex with 3'3'-c-di-AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 07A |
| Synchrotron site | NSRRC |
| Beamline | TPS 07A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.171, 52.713, 88.932 |
| Unit cell angles | 90.00, 114.88, 90.00 |
Refinement procedure
| Resolution | 25.769 - 2.553 |
| Rwork | 0.195 |
| R-free | 0.22760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.438 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP (11.7.03) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.121 | 0.578 |
| Number of reflections | 20886 | 1948 |
| <I/σ(I)> | 14.1 | 2.3 |
| Completeness [%] | 96.5 | |
| Redundancy | 6.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M MES pH 6.0, 0.1 M Sodium chloride, 30 % v/v PEG 3500 |
