8HU6
AMP deaminase 2 in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 123.665, 163.563, 289.385 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.350 - 2.330 |
| R-factor | 0.2383 |
| Rwork | 0.237 |
| R-free | 0.26750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a3l |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.506 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 289.390 | 2.390 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.080 | 0.373 |
| Rmeas | 0.094 | 0.420 |
| Rpim | 0.049 | 0.189 |
| Number of reflections | 122956 | 8943 |
| <I/σ(I)> | 12.1 | 3.1 |
| Completeness [%] | 99.0 | |
| Redundancy | 4.1 | |
| CC(1/2) | 0.980 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 295.15 | 85 mM MES ph 5.9, 20% PEG8000, 170 mM ammonium sulfate, 15% glycerol, 10mM AMP |
