8HS8

a bacteria protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-22
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.979
Spacegroup name	P 31
Unit cell lengths	129.504, 129.504, 44.546
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.280 - 2.700
R-factor	0.2693
Rwork	0.268
R-free	0.30380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xvq 6jdq
RMSD bond length	0.005
RMSD bond angle	0.787
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.720	2.796
High resolution limit [Å]	2.700	2.700
Number of reflections	1155940	2720
<I/σ(I)>	15.6
Completeness [%]	99.1
Redundancy	9.9
CC(1/2)	0.979	0.482

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	PEG 6000, MES/ Sodium hydroxide, Ammonium chloride