8HRV
dutpase of helicobacter pylori 26695
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9805 |
| Spacegroup name | P 63 |
| Unit cell lengths | 103.869, 103.869, 78.651 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.280 - 2.000 |
| Rwork | 0.145 |
| R-free | 0.18120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.442 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.950 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.129 | |
| Number of reflections | 32696 | 2380 |
| <I/σ(I)> | 18 | |
| Completeness [%] | 100.0 | |
| Redundancy | 19.3 | |
| CC(1/2) | 0.990 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.1 M amino acid mix,0.1 M buffer system1 pH 6.5, 50% v/v precipitant mix4 |
