8HRQ
Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum ATCC13032 (L36S/T37K) in complex with NAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-10-31 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.710, 117.891, 77.128 |
Unit cell angles | 90.00, 116.92, 90.00 |
Refinement procedure
Resolution | 26.710 - 2.090 |
R-factor | 0.18023 |
Rwork | 0.177 |
R-free | 0.24550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8hro |
RMSD bond length | 0.008 |
RMSD bond angle | 1.591 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
High resolution limit [Å] | 2.090 | 5.700 | 2.090 |
Rmerge | 0.991 | 0.037 | 0.240 |
Number of reflections | 39134 | 3795 | 3606 |
<I/σ(I)> | 18.3 | ||
Completeness [%] | 92.1 | 91.9 | 87.1 |
Redundancy | 1.7 | 1.8 | 1.6 |
CC(1/2) | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3350, Cesium chloride |