8HRH
SN-131/1B2 anti-MUC1 antibody with a glycopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-07 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 84.590, 84.590, 271.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.766 - 2.070 |
| Rwork | 0.192 |
| R-free | 0.22650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kx1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.879 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16-3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.766 | 2.200 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmeas | 0.127 | 0.758 |
| Number of reflections | 36078 | 5649 |
| <I/σ(I)> | 16.6 | 3.43 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 19.9 | 20.3 |
| CC(1/2) | 0.999 | 0.956 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 40% (w/v) PEG300, 5% (w/v) PEG1000, 0.1M Tris |
