8HQX
The complex structure of COPI cargo sorting module with TAT-WDM peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.863, 50.831, 72.643 |
Unit cell angles | 90.00, 93.97, 90.00 |
Refinement procedure
Resolution | 36.495 - 1.538 |
R-factor | 0.2248 |
Rwork | 0.224 |
R-free | 0.23940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.739 |
Data reduction software | HKL-3000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.16.3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.600 |
High resolution limit [Å] | 1.538 | 1.550 |
Rmerge | 0.086 | 0.414 |
Number of reflections | 28943 | 240 |
<I/σ(I)> | 12.2 | |
Completeness [%] | 98.2 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 281.15 | 0.1 M MES, pH 6.5 and 20% PEG3350 |