8HQV
The complex structure of COPI cargo sorting module with HCoV-OC43 Spike KTSHxx sorting motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.930, 51.273, 85.256 |
Unit cell angles | 90.00, 101.61, 90.00 |
Refinement procedure
Resolution | 42.033 - 1.398 |
R-factor | 0.1876 |
Rwork | 0.187 |
R-free | 0.20780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.250 |
Data reduction software | HKL-3000 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.16.3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.500 |
High resolution limit [Å] | 1.398 | 1.400 |
Rmerge | 0.089 | |
Number of reflections | 56731 | 4767 |
<I/σ(I)> | 12.6 | |
Completeness [%] | 80.2 | 80.2 |
Redundancy | 3.7 | 3.2 |
CC(1/2) | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 281.15 | 0.2 M MgSO4 and 20% PEG3350 |