8HQ4

B27 in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-10-24
Detector	MAR CCD 130 mm
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	106.080, 106.080, 304.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.060 - 2.120
R-factor	0.1879
Rwork	0.187
R-free	0.21020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.432
Data reduction software	HKL-2000
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.060	32.060	2.180
High resolution limit [Å]	2.120	9.480	2.120
Rmerge	0.133	0.079	2.140
Rmeas	0.136	0.081	2.180
Rpim	0.027	0.017	0.412
Total number of observations	2605400	26161	198874
Number of reflections	99275	1272	7240
<I/σ(I)>	16.5	37.5	2.1
Completeness [%]	99.9	97.5	100
Redundancy	26.2	20.6	27.5
CC(1/2)	0.999	0.997	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.12 M Monosaccharides (20 mM D-Glucose; 20 mM D-Mannose; 20 mM D-Galactose; 20 mM L-Fucose; 20 mM D-Xylose; 20 mM N-Acetyl-D-Glucosamine), 0.1 M buffer system 1 pH 6.5 (sodium HEPES and MOPS), and 50 % Precipitant Mix 2 (40% v/v Ethylene glycol; 20 % w/v PEG 8000)