8HP8

Crystal Structure of Engineered Endolysin EC340 derived from Gram-Negative Bacteria targeted Bacteriophage

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	83.700, 125.042, 69.237
Unit cell angles	90.00, 114.74, 90.00

Refinement procedure

Resolution	34.780 - 2.397
R-factor	0.1807
Rwork	0.176
R-free	0.23600
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.814
Data scaling software	HKL-2000
Phasing software	PHENIX ((1.14_3260))
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.780	6.540
High resolution limit [Å]	2.397	2.400
Number of reflections	24857	1197
<I/σ(I)>	25.3529
Completeness [%]	98.3
Redundancy	6.8
CC(1/2)	0.975	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1M citrate, pH 5.0, 20% w/v PEG 6000