8HO6
ScRIPK WT
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-10 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.170, 72.350, 79.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.738 - 2.100 |
R-factor | 0.2059 |
Rwork | 0.203 |
R-free | 0.23360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tos |
RMSD bond length | 0.007 |
RMSD bond angle | 0.962 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.060 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.138 | |
Number of reflections | 23842 | 23842 |
<I/σ(I)> | 13 | 1.7 |
Completeness [%] | 99.7 | |
Redundancy | 12.79 | |
CC(1/2) | 0.998 | 0.853 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M HEPES pH 7.5, 40% v/v Polyethylene glycol 400, 0.19 mM Cymal-7, 10% v/v Polyethylene glycol 3350, 0.18 mM GDN |