8HO2
crystal structure of Norcoclaurine synthase from Chinese lotus (Nelumbo nucicera)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.262, 58.104, 114.139 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.379 - 1.711 |
| R-factor | 0.1579 |
| Rwork | 0.157 |
| R-free | 0.17900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5non |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (2.7.0) |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.379 | 1.732 |
| High resolution limit [Å] | 1.711 | 1.711 |
| Rmerge | 0.121 | |
| Rmeas | 0.126 | |
| Rpim | 0.035 | 0.104 |
| Number of reflections | 64909 | 34476 |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 99.1 | |
| Redundancy | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 300 | 12.8 mM (NH4)2SO4, 8% PEG-3350, 10 mM betaine hydrochloride |
