8HM2
Crystal structure of human ubiquitin-like protein from bacteroides fragilis c terminal cysteine mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-20 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 38.537, 38.537, 177.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.070 - 1.340 |
R-factor | 0.2562 |
Rwork | 0.256 |
R-free | 0.26810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.007 |
RMSD bond angle | 1.067 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.070 | 1.388 |
High resolution limit [Å] | 1.340 | 1.340 |
Number of reflections | 30343 | 2397 |
<I/σ(I)> | 13.5 | |
Completeness [%] | 96.8 | |
Redundancy | 20 | |
CC(1/2) | 0.994 | 0.697 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 2.8 | 291 | 0.1M citric acid pH 2.8, 1.6 M ammonium sulfate |