8HLO
Crystal structure of ASAP1-SH3 and MICAL1-PRM complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 62 |
| Unit cell lengths | 45.181, 45.181, 59.391 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.128 - 1.168 |
| R-factor | 0.1143 |
| Rwork | 0.111 |
| R-free | 0.14240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.317 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASES |
| Refinement software | PHENIX (v1.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.128 | 1.210 |
| High resolution limit [Å] | 1.168 | 1.170 |
| Rmerge | 0.041 | |
| Number of reflections | 21440 | 1130 |
| <I/σ(I)> | 11.32 | |
| Completeness [%] | 91.7 | |
| Redundancy | 4.9 | |
| CC(1/2) | 0.990 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 289.15 | 0.1M HEPES, pH 7.5, 1.4M Sodium citrate tribasic dihydrate |
