8HLN
Crystal structure of p53/BCL2 fusion complex(complex3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-19 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.701, 70.528, 127.725 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.829 - 2.354 |
| R-factor | 0.2071 |
| Rwork | 0.202 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1TUP/6QGG |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.541 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
| Rmerge | 0.169 | 0.098 | 0.672 |
| Rmeas | 0.177 | 0.102 | 0.711 |
| Rpim | 0.051 | 0.029 | 0.225 |
| Total number of observations | 180047 | ||
| Number of reflections | 16906 | 963 | 673 |
| <I/σ(I)> | 7.8 | ||
| Completeness [%] | 97.1 | 99.1 | 81.3 |
| Redundancy | 10.6 | 11.8 | 7.3 |
| CC(1/2) | 0.996 | 0.912 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | 0.1 M HEPES, PEG8000 8%-13%, 0.8% ethylene glycol |
