8HLM

Crystal structure of p53/BCL2 fusion complex (complex 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-19
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	45.610, 70.336, 127.158
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.205 - 2.522
R-factor	0.2394
Rwork	0.234
R-free	0.29010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1TUP/6QGG
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.15.2_3472)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.580	2.612
High resolution limit [Å]	2.520	2.522
Rmerge	0.088	0.911
Rpim		0.308
Number of reflections	13872	1383
<I/σ(I)>	0.0935
Completeness [%]	96.2
Redundancy	9.1
CC(1/2)	0.999	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	291	0.1 M Imidazole, PEG8K 15-21%