8HLL
Crystal structure of p53/BCL2 fusion complex (complex 1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-01-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.870, 70.480, 126.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.624 - 2.620 |
R-factor | 0.2302 |
Rwork | 0.225 |
R-free | 0.27950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1TUP/6QGG |
RMSD bond length | 0.003 |
RMSD bond angle | 0.550 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.624 | 2.714 |
High resolution limit [Å] | 2.620 | 2.620 |
Rmerge | 0.257 | 1.185 |
Rmeas | 0.268 | |
Rpim | 0.075 | 0.341 |
Number of reflections | 13228 | 1299 |
<I/σ(I)> | 39.74 | 2.83 |
Completeness [%] | 99.7 | 99.85 |
Redundancy | 12.9 | 13 |
CC(1/2) | 0.977 | 0.867 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | PEG 8K 8%-13%, 0.1M HEPES, 0.8% ethylene glycol |