8HL9

Crystal structure of Galectin-8 C-CRD with part of linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 41 21 2
Unit cell lengths	52.900, 52.900, 126.749
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.620 - 1.600
R-factor	0.2166
Rwork	0.213
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.910
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (v1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.620	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.058	0.520
Number of reflections	24410	1173
<I/σ(I)>	13.1
Completeness [%]	99.3
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Hepes pH 7.0, 0.2M MgCl2, 30% (W/V) PEG 3350