8HKG
Crystal structure of glycosidic hydrolase family 10 (GH10) xylanase XynA contains an additional proline-rich sequence in the C-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.922, 99.461, 87.833 |
| Unit cell angles | 90.00, 100.91, 90.00 |
Refinement procedure
| Resolution | 24.240 - 2.560 |
| R-factor | 0.2192 |
| Rwork | 0.215 |
| R-free | 0.30830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.111 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.240 | 2.655 |
| High resolution limit [Å] | 2.563 | 2.563 |
| Rmerge | 0.088 | |
| Number of reflections | 18264 | |
| <I/σ(I)> | 6.57 | |
| Completeness [%] | 98.9 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.969 | 0.969 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 30% (w/v) PEG 4000, 0.2M ammonium acetate, 0.1M sodium acetate trihydrate |
