8HJF
Crystal structure of glycosyltransferase SgUGT94-289-3 in complex with M5, state 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.955, 76.814, 81.459 |
Unit cell angles | 90.00, 93.25, 90.00 |
Refinement procedure
Resolution | 19.660 - 2.000 |
R-factor | 0.1996 |
Rwork | 0.198 |
R-free | 0.24030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jtd |
RMSD bond length | 0.005 |
RMSD bond angle | 0.972 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.660 | 2.071 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 64729 | 6412 |
<I/σ(I)> | 5.9 | |
Completeness [%] | 99.6 | |
Redundancy | 6.8 | |
CC(1/2) | 0.987 | 0.460 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | Tris-HCl, polyethylene glycol 3350, NaCl, Polypropylene glycol P400. |