8HIG

Co-crystal structure of C-terminal DNA binding domain of Saccharopolyspora erythraea GlnR in complex with its cognate promoter DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 31 2 1
Unit cell lengths	46.279, 46.279, 366.859
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.842 - 2.329
R-factor	0.2654
Rwork	0.259
R-free	0.32470
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.329	2.340
Rmerge		1.720
Number of reflections	19059	569
<I/σ(I)>	11.146	2.034
Completeness [%]	90.7	78.3
Redundancy	5.1
CC(1/2)	0.900	0.107

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.1M Bis-Tris pH5.7, 25% PEG3350