8HIB

The crystal structure of Pygo2-LDB1-SSBP2 triple complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 65 2 2
Unit cell lengths	104.499, 104.499, 252.274
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.920 - 2.450
R-factor	0.2146
Rwork	0.213
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tyd
RMSD bond length	0.010
RMSD bond angle	1.110
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.19.2-4158)
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.520
High resolution limit [Å]	2.450	2.450
Number of reflections	30828	2491
<I/σ(I)>	44.86
Completeness [%]	100.0
Redundancy	12.7
CC(1/2)	0.998	0.614

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM Li2SO4, 100 mM sodium citrate tribasic dihydrate pH 5.6, 1% v/v PEG400, and 10 mM DTT