8HHJ

Crystal structure of the MafB2-CTMGI-2B16B6/MafI2MGI-2B16B6 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2000-03-30
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9734
Spacegroup name	C 1 2 1
Unit cell lengths	78.667, 39.792, 65.547
Unit cell angles	90.00, 109.16, 90.00

Refinement procedure

Resolution	28.903 - 1.500
R-factor	0.1822
Rwork	0.180
R-free	0.20820
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.808
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX (v1.11)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	30286	1523
<I/σ(I)>	48.3
Completeness [%]	97.9
Redundancy	7.3
CC(1/2)	0.999	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.25 - 0.3 M magnesium formate, 25 - 30% polyethylene glycol (PEG) 3350