8HGI
Crystal structure of VNAR aGFP14 in complex with GFP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-26 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97902 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.449, 102.797, 66.620 |
| Unit cell angles | 90.00, 116.33, 90.00 |
Refinement procedure
| Resolution | 59.710 - 1.950 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ppk 7fbj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.514 |
| Data reduction software | DIALS (0.7.7) |
| Data scaling software | SCALEPACK (0.5.28) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.800 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.072 | 0.523 |
| Number of reflections | 51988 | |
| <I/σ(I)> | 10.3 | 2.5 |
| Completeness [%] | 99.4 | 92.7 |
| Redundancy | 5.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 291 | 18%(w/v) PEG8000 0.1M Na/K PO4 pH6.8 |
