8HDD
Complex structure of catalytic, small, and a partial electron transfer subunits from Burkholderia cepacia FAD glucose dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 204.015, 71.797, 114.221 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.880 - 3.000 |
R-factor | 0.27778 |
Rwork | 0.275 |
R-free | 0.32335 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6a2u |
RMSD bond length | 0.002 |
RMSD bond angle | 1.135 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.080 |
High resolution limit [Å] | 3.000 | 13.420 | 3.000 |
Rmerge | 0.165 | 0.030 | 0.732 |
Rmeas | 0.179 | 0.033 | 0.791 |
Number of reflections | 34334 | 447 | 2443 |
<I/σ(I)> | 10.34 | ||
Completeness [%] | 99.8 | ||
Redundancy | 6.7 | ||
CC(1/2) | 0.998 | 1.000 | 0.961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | COUNTER-DIFFUSION | 5.6 | 293 | tacsimate, PEG 3350, DDM, sodium citrate |