8HD5

The crystal structure of Hu protein in Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-18
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	41.427, 82.369, 61.633
Unit cell angles	90.00, 93.16, 90.00

Refinement procedure

Resolution	29.190 - 2.140
R-factor	0.2056
Rwork	0.203
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.884
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.190	2.220
High resolution limit [Å]	2.140	2.140
Number of reflections	22354	2190
<I/σ(I)>	12.62
Completeness [%]	98.0
Redundancy	2
CC(1/2)	0.998	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	296	0.1M MES, 15% PEG 3350