8HD2

Crystal structure of SAM dependent methyltransferase encoded in type II fatty acid biosynthesis gene cluster from ladderane lipid producing anammox bacteria

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	35.016, 48.060, 49.828
Unit cell angles	82.59, 84.12, 70.67

Refinement procedure

Resolution	45.120 - 1.600
R-factor	0.1814
Rwork	0.180
R-free	0.20780
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.852
Data reduction software	DIALS
Data scaling software	pointless
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.120	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	38897	1761
<I/σ(I)>	7
Completeness [%]	97.1
Redundancy	3.4
CC(1/2)	0.994	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	15 % PEG 2000, 0.1 M HEPES