8HCL
zebrafish IRF-10 DBD complex with DNA
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 298.15 |
Detector technology | CCD |
Collection date | 2020-10-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.010, 86.470, 57.150 |
Unit cell angles | 90.00, 103.97, 90.00 |
Refinement procedure
Resolution | 38.830 - 1.990 |
R-factor | 0.2254 |
Rwork | 0.223 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2irf |
RMSD bond length | 0.011 |
RMSD bond angle | 1.508 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.460 | 8.900 |
High resolution limit [Å] | 1.990 | 1.990 |
Number of reflections | 25670 | 1894 |
<I/σ(I)> | 12.6 | |
Completeness [%] | 98.8 | 99 |
Redundancy | 3.4 | 2.3 |
CC(1/2) | 0.996 | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.1M sodium acetate trihydrate pH 4.5, 25% polyethylene glycol 3350 |