8HCG

Crystal structure of mTREX1-dAMP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2020-08-03
Detector	RAYONIX MX300-HS
Wavelength(s)	0.99984
Spacegroup name	P 32 2 1
Unit cell lengths	92.585, 92.585, 67.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.643 - 1.799
R-factor	0.1669
Rwork	0.165
R-free	0.19160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yws
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.860
High resolution limit [Å]	1.799	3.880	1.800
Rmerge	0.071	0.048	0.487
Rmeas	0.076	0.052	0.521
Rpim	0.025	0.018	0.182
Number of reflections	31351	3274	3081
<I/σ(I)>	11
Completeness [%]	99.9	99.5	99.9
Redundancy	9.2	8.4	7.8
CC(1/2)		0.998	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES pH 7.5, 40% v/v Polyethylene glycol 400