8HC0
Crystal structure of the extracellular domains of GPR110
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL10U2 |
Synchrotron site | SSRF |
Beamline | BL10U2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 134.825, 47.993, 87.023 |
Unit cell angles | 90.00, 127.35, 90.00 |
Refinement procedure
Resolution | 19.800 - 2.900 |
R-factor | 0.2355 |
Rwork | 0.233 |
R-free | 0.29180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | predicted by AlphaFold2 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.579 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (phenix-1.19.2-4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.800 | 43.800 | 3.070 |
High resolution limit [Å] | 2.900 | 8.540 | 2.900 |
Rmerge | 0.124 | 0.049 | 0.579 |
Rmeas | 0.149 | 0.060 | 0.701 |
Number of reflections | 18390 | 409 | 1555 |
<I/σ(I)> | 9.68 | ||
Completeness [%] | 98.0 | ||
Redundancy | 3.18 | ||
CC(1/2) | 0.989 | 0.995 | 0.732 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 289 | 0.2 M ammonium sulfate, 0.1 M sodium acetate trihydrate pH 4.6, 30% PEG-MME 2,000, 15% glycerol |