8HAQ
The complex of Src with GW8510
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2022-09-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97914 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.830, 62.580, 74.260 |
| Unit cell angles | 79.30, 87.98, 89.73 |
Refinement procedure
| Resolution | 32.960 - 2.270 |
| R-factor | 0.2095 |
| Rwork | 0.207 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bdf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.288 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.200 | 2.330 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rpim | 0.131 | 0.644 |
| Number of reflections | 33316 | 2502 |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 97.5 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MOPS pH 7.0, 18% PEG 3350, 5% Glycerol, 5 mM TCEP |
