8H8H
Crystal structure of the N-terminal domain of H-NS family protein TurB (TurB_nt50)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2018-06-09 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.692, 63.303, 275.872 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.920 - 2.700 |
| R-factor | 0.2505 |
| Rwork | 0.246 |
| R-free | 0.28510 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.074 |
| Data reduction software | XDS (Jan 10, 2022) |
| Data scaling software | Aimless (CCP4Interface 8.0) |
| Phasing software | CRANK2 (CCP4Interface 7.0) |
| Refinement software | PHENIX ((1.20.1_4487)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.640 | 3.180 | 2.830 |
| High resolution limit [Å] | 2.700 | 3.000 | 2.700 |
| Rmerge | 0.140 | 0.471 | 1.355 |
| Rmeas | 0.146 | 0.481 | |
| Rpim | 0.041 | 0.095 | 0.375 |
| Number of reflections | 22936 | 2447 | 2231 |
| <I/σ(I)> | 10.9 | 5.5 | 2.4 |
| Completeness [%] | 98.9 | 92.4 | 98.75 |
| Redundancy | 13.1 | 13.8 | |
| CC(1/2) | 0.996 | 0.956 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 279 | PEG 3350, HEPES, ammonium acetate |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 279 | PEG 3350, HEPES, ammonium acetate |
