8H7T

Trans-3/4-proline-hydroxylase H11 apo structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	106.600, 106.600, 143.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.780 - 1.880
R-factor	0.1359
Rwork	0.133
R-free	0.18360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nci
RMSD bond length	0.003
RMSD bond angle	0.953
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.880	1.880
Rmerge	0.133
Number of reflections	64234	4933
<I/σ(I)>	25.3	5.48
Completeness [%]	99.7	99.8
Redundancy	15.2
CC(1/2)		0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289.15	0.7M Sodium citrate tribasic dihydrate, 0.1M Bis-Tris propane (pH 7.0)