8H1M

Crystal structure of glucose-2-epimerase mutant_D254A from Runella slithyformis Runsl_4512

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-10-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000000
Spacegroup name	P 43 21 2
Unit cell lengths	113.219, 113.219, 116.284
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.040 - 1.600
R-factor	0.1451
Rwork	0.144
R-free	0.16620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.525
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.040	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.083
Number of reflections	99920	9806
<I/σ(I)>	20.83
Completeness [%]	99.9
Redundancy	13.1
CC(1/2)	0.999	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	3.6M sodium formate