8H1K

Crystal structure of glucose-2-epimerase from Runella slithyformis Runsl_4512

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-06-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000000
Spacegroup name	P 43 21 2
Unit cell lengths	113.366, 113.366, 116.031
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.000 - 1.600
R-factor	0.1428
Rwork	0.142
R-free	0.16160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vw5
RMSD bond length	0.018
RMSD bond angle	1.570
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	1.700
High resolution limit [Å]	1.600	1.600
Rmerge	0.150	0.871
Number of reflections	99680	9841
<I/σ(I)>	14.15
Completeness [%]	100.0
Redundancy	13.2
CC(1/2)	0.997	0.923

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	3.4 M sodium formate, 20 mM glucose