8H0J

Annexin A5 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	69.294, 134.470, 79.227
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.630 - 2.230
R-factor	0.1907
Rwork	0.188
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1avh
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.290
High resolution limit [Å]	2.230	6.090	2.250
Rmerge	0.166	0.106	1.018
Rmeas	0.173	0.111	1.079
Rpim	0.049	0.033	0.349
Number of reflections	18237	998	884
<I/σ(I)>	3.2
Completeness [%]	100.0	100	99.8
Redundancy	12.4	11.7	9
CC(1/2)		0.994	0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	PEG 3350, sodium chloride, 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid