8H0E
Crystal structure of collagen heterotrimer with KD, ER and KE axial pairs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 61.067, 42.411, 28.749 |
Unit cell angles | 90.00, 106.59, 90.00 |
Refinement procedure
Resolution | 29.260 - 1.760 |
R-factor | 0.2261 |
Rwork | 0.225 |
R-free | 0.25770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yan |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.260 | 1.800 |
High resolution limit [Å] | 1.760 | 1.770 |
Rmerge | 0.103 | 0.103 |
Number of reflections | 6945 | 6884 |
<I/σ(I)> | 22.47 | |
Completeness [%] | 96.6 | |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10% isopropanol, 0.1M Bicine pH 8.5, 30% PEG3350 |