8GZE

Crystal Structure of human METTL9-SAH-SLC39A7 peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 61 2 2
Unit cell lengths	79.182, 79.182, 422.365
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.280 - 3.400
R-factor	0.26
Rwork	0.255
R-free	0.30450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ezg
RMSD bond length	0.003
RMSD bond angle	0.775
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.460
High resolution limit [Å]	3.400	9.210	3.400
Rmerge	0.966	0.057	0.687
Total number of observations	56335
Number of reflections	18922	1050	919
<I/σ(I)>	3.4
Completeness [%]	92.4	99.4	89
Redundancy	3	4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	20% w/v PEG 4000, 500 mM KCl.