8GZC

Crystal structure of EP300 HAT domain in complex with compound 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-22
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.100000
Spacegroup name	P 1
Unit cell lengths	43.500, 89.350, 91.600
Unit cell angles	116.60, 95.18, 91.78

Refinement procedure

Resolution	19.910 - 2.000
R-factor	0.1961
Rwork	0.195
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vi0
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.910	19.910	2.040
High resolution limit [Å]	1.990	8.900	1.990
Rmerge	0.096	0.032	1.745
Rmeas	0.118	0.039	2.468
Total number of observations	424926
Number of reflections	82690	1809	10151
<I/σ(I)>	5.74	21.4	0.29
Completeness [%]	96.8	95.3	81.7
Redundancy	2.604	2.812	1.536
CC(1/2)	0.996	0.997	0.167

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	15% PEG3350, 0.1 M HEPES